microwell slide a10657-01 fcs unit c9413 series inverted microscope notes: sonication should be limited to one time only. (sonica- tion time cannot exceed 15 minutes.) please handle with care when cleaning the microwell slide in a beaker or some other glass container, as cracks may form on the microwell slide upon contact with a glass surface. information furnished by hamamatsu is believed to be reliable. however, no responsibility is assumed for possible inaccuracies or omissions. specifications are subject to change without notice. no patent rights are granted to any of the circuits described herein. ?009 hamamatsu photoni cs k.k. subject to local technical requirements and regulations, availability of products included in this promotional material may var y. please consult with our sales office. specifications dimensional outline (unit: mm) features application examples material number of wells recommended working volume well diameter well depth cover glass thickness refractive index of cover glass dimensions sterile package slide glass cover glass soda-lime glass borosilicate glass 21 (3 7) 25 l 4 mm (top), 3 mm (bottom) 3.1 mm 0.12 mm to 0.17 mm 1.525 57.2 mm 25.4 mm 3.4 mm untreated * 10 slides description / value parameter note: * max. autoclaving cycle: 3 times (121 c, 103 kpa, 20 minutes) totha0003ea small well volume (25 l) for conserving samples or re- agents optical grade bottom cover glass that allows measure- ment with an inverted microscope alphanumeric grid for easy sample identification background fluorescence is minimized by the glass suitable for fluorescence correlation spectroscopy (fcs) b c 1234567 a 25.4 p5.0 6=30.0 2.0 5.0 2.0 5.0 57.2 24.0 50.0 4.0 3.0 3.4 10 3-r1.0 r1.0 r1.0 cover glass
toth1004e03 apr. 2009 ip microwell slide a10657-01 hamamatsu photonics k.k., electron tube division 314-5, shimokanzo, iwata city, shizuoka pref., 438-0193, japan, telephone: (81)539/62-5248, fax: (81)539/62-2205 u.s.a.: hamamatsu corporation: 360 foothill road, p. o. box 6910, bridgewater. n.j. 08807-0910, u.s.a., telephone: (1)908-231-0960, fa x: (1)908-231-1218 e-mail: usa@hamamatsu.com germany: hamamatsu photonics deutschland gmbh: arzbergerstr. 10, d-82211 herrsching am ammersee, germany, telephone: (49)8152-375-0, f ax: (49)8152-2658 e-mail: info@hamamatsu.de france: hamamatsu photonics france s.a.r.l.: 19, rue du saule trapu, parc du moulin de massy, 91882 massy cedex, france, telephone: (3 3)1 69 53 71 00, fax: (33)1 69 53 71 10 e-mail: infos@hamamatsu.fr united kingdom: hamamatsu photonics uk limited: 2 howard court, 10 tewin road welwyn garden city hertfordshire al7 1bw, united kingdom, teleph one: 44-(0)1707-294888, fax: 44(0)1707-325777 e-mail: info@hamamatsu.co.uk north europe: hamamatsu photonics norden ab: smidesv?gen 12, se-171-41 solna, sweden, telephone: (46)8-509-031-00, fax: (46)8-509-031-01 e -mail: info@hamamatsu.se italy: hamamatsu photonics italia: s.r.l.: strada della moia, 1/e, 20020 arese, (milano), italy, telephone: (39)02-935 81 733, fax: (39)02-935 81 741 e-mail: info@hamamatsu.it web site www.hamamatsu.com measurement examples measurement example 1: egfp expressing and non-expressing hela cell stained with mitotracker deep red 633 fm measurement example 2: fcs measurement in hela cell courtesy of mr. keishi sakata, ms. makiyo uchida, prof. masataka kinjo, laboratory of molecular cell dynamics, faculty of advanced life science, graduate school of life science, hokkaido university system: confocal laser scanning microscope sample: hela cell (egfp expressing and non-expressing) orthochromatic dye: mitotracker deep red 633 fm (molecular probes, m22426) (ex 644 nm, em 665 nm) staining conditions: 100 nm in opti-mem, 37 c, 5 %co 2 , 20 min system: confocal laser scanning microscope sample: egfp expressing hela cell (cell cytoplasm) excitation light: ar + laser: 488 nm fm fluorescence filter: 500 nm to 550 nm measurement time: 15 s 5 times c-apo 40 /1.2 w hft uv/488/543/633 nft 635 vis bp 500-550 ir laser he-ne 633 nm 3 % ar 488 nm 1 % lp 650 0510 time (s) intensity ( 10 5 s -1 ) 15 4 4.5 5 10 0 10 2 10 4 ( s) g ( ) 10 6 1.000 1.005 1.010 hft: main dichroic beam spliter nft: secondary dichroic beam spliter bp : band pass filter lp : long pass filter
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